In this study four conventional parasitological methods like Wet blood film (WBF), thin and thick blood smear and Buffy coat technique (BCT) were compared for diagnostic sensitivity of Trypanosoma evansi in naturally infected buffaloes. Out of 250 field blood samples collected from different places of Indore and Ratlam district of M.P., 1.2% were found positive by wet blood film, 4.4% by thin blood smear, 9.2% by thick blood smear and 14.8% by buffy coat technique for T. evansi. The sensitivity and specificity of the all methods were analyzed and it was observed that BCT is more sensitive than the other conventional methods of examination.

Highlights

• Conventional parasitological methods were compared for diagnostic sensitivity of Trypanosoma evansi in naturally infected buffaloes.
• Buffy coat technique is more sensitive than the other conventional methods of examination.